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常见荧光染料峰激发波长和发射波长
2024.09.23   点击396次

荧光染料通常溶于有机溶剂或水溶液中。不同溶剂的极性不同,染料分子在不同溶剂中的环境也不同,导致荧光发射峰的偏移的情况发生。因此建议使用前咨询染料制造商来确认特定荧光染料的数据,以确保激发和发射曲线的完整。

荧光染料激发波长发射波长
3-Hydroxypyrene-5,-8,10-TriSulfonic Acid403513
5-(and 6-)carboxy SNARF-1 indicator548(low pH)587(low pH)
5-(and 6-)carboxy SNARF-1 indicator576(high pH)635(high pH)
5-Hydroxy-Tryptamine (5-HT)380-415520-530
6-Carboxyrhodamine 6G525555
7-Aminoactinomycin D (7-AAD) †546647
7-Hydroxy-4-methylcoumarin360455
Acid Fuchsin540630
ACMA430474
Acridine orange500 (+DNA)526 (+DNA)
Acridine orange460 (+RNA)650 (+RNA)
Acridine Orange (Bound to DNA)502526
Acridine Red455-600560-680
Acridine Yellow470550
Acriflavin436520
AFA (Acriflavin Feulgen SITSA)355-425460
Alexa Fluor 350 dye, 7-amino-4-methylcoumarin (AMC)346442
Alexa Fluor 405 dye402421
Alexa Fluor 430 dye434539
Alexa Fluor 488 dye495519
Alexa Fluor 514 dye518540
Alexa Fluor 532 dye531554
Alexa Fluor 546 dye556573
Alexa Fluor 555 dye555565
Alexa Fluor 568 dye578603
Alexa Fluor 594 dye590617
Alexa Fluor 610 dye612628
Alexa Fluor 633 dye632647
Alexa Fluor 635 dye633647
Alexa Fluor 647 dye650668
Alexa Fluor 660 dye663690
Alexa Fluor 680 dye679702
Alexa Fluor 700 dye702723
Alexa Fluor 750 dye749775
Alexa Fluor 790 dye782805
Alizarin Complexon530-560580
Alizarin Red530-560580
Allophycocyanin650660
AMCA-S, AMC345445
Aminoactinomycin D555655
Aminocoumarin350445
Anthroyl Stearate361-381446
Astrazon Brilliant Red 4G500585
Astrazon Orange R470540
Astrazon Red 6B520595
Astrazon Yellow 7 GLL450480
Atabrine436490
Auramine460550
Aurophosphine450-490515
Aurophosphine G450580
BAO 9-(Bisamino-phenyloxadiazole)365395
BCECF505530
BCECF indicator482 (low pH)520 (low pH)
BCECF indicator503 (high pH)528 (high pH)
Berberine Sulphate430550
Bimane380458
Bisbenzamide360600-610
Blancophor FFG Solution390470
Blancophor SV370435
BOBO-1, BO-PRO-1462481
BODIPY 630/650 dye630650
BODIPY 650/665 dye650665
BODIPY FL dye505513
BODIPY TMR-X dye542574
BODIPY TR-X dye589617
BOPRO 1462481
Brilliant Sulpho-flavin FF430520
Calcein494517
Calcien Blue370435
Calcium Crimson indicator590615
Calcium Green indicators506533
Calcium Orange indicator549576
Calcofluor RW Solution370440
Calcofluor White440500-520
Calcofluor White ABT Solution380475
Calcofluor White Standard Solution365435
Carboxy SNARF indicators548 (low pH)587 (low pH)
Carboxy SNARF indicators576 (high pH)635 (high pH)
Cascade Blue dye400420
Cascade Yellow dye402545
Catecholamine410470
Chinacrine450-490515
CL-NERF504(low pH)587(low pH)
CL-NERF514(high pH)540(high pH)
Coriphosphine O460575
Coumarin-Phalloidin387470
CY3.18554568
CY5.18649666
CY7710805
DANS (1-DimethylAmino-Naphthaline-5-Sulphonic Acid)340525
DANSA (DiaminoNaphthyl-Sulphonic Acid)340-380430
Dansyl NH-CH3 in water340578
DAPI †358461
Di-8-ANEPPS488605
Di-8-ANEPPS, Di-4-ANEPPS ‡468635
DiA ‡456590
Diamino Phenyl Oxydiazole (DAO)280460
DiD (DiIC18(5))644665
DiI (DiIC18(3))549565
Dimethylamino-5-Sulphonic Acid310-370520
DiO (DiOC18(3))484501
Diphenyl Brilliant Flavine 7GFF430520
DiR (DiIC18(7))750779
DM-NERF497(low pH)527(low pH)
DM-NERF510(high pH)536(high pH)
Dopamine340490-520
ELF 97 alcohol345530
Eosin524544
ER Tracker Blue-White DPX ‡372556
Erythrosin ITC530558
EthD-1 †528617
Ethidium bromide †518605
Euchrysin430540
FIF (Formaldehyde Induced Fluorescence)405435
Flazo Orange375-530612
Fluo-3 indicator506526
Fluo-4 indicator494516
Fluorescein494518
Fluorescein Iso-thiocyanate (FITC)490525
Fluorescein, FITC494518
FM 1-43, FM 1-43FX ‡479598
FM 4-64, FM 4-64FX ‡506750
FM1-43479598
Fura Red indicator472 (low [Ca2+])657 (low [Ca2+])
Fura Red indicator436 (high [Ca2+])637 (high [Ca2+])
Fura-2 indicator363 (low [Ca2+])512 (low [Ca2+])
Fura-2 indicator335 (high [Ca2+])505 (high [Ca2+])
Genacryl Brilliant Red B520590
Genacryl Brilliant Yellow 10GF430485
Genacryl Pink 3G470583
Genacryl Yellow 5GF430475
Gloxalic Acid405460
Granular Blue355425
Haematoporphyrin530-560580
Hoechst 33258, Hoechst 33342 †352461
Indo-1 indicator346 (low [Ca2+])475 (low [Ca2+])
Indo-1 indicator330 (high [Ca2+])401 (high [Ca2+])
Intrawhite Cf Liquid360430
JC-1498 (monomer)525 (monomer)
JC-1593 (aggregate)595 (aggregate)
JC-9506525 (monomer)
JC-9506635 (aggregate)
JOE dye525555
Leucophor PAF370430
Leucophor SF380465
Leucophor WS395465
Lissamine rhodamine B570590
Lissamine Rhodamine B200 (RD200)575595
Lucifer yellow CH428536
Lucifer Yellow VS430535
LysoSensor Blue DND-167374425
LysoSensor Blue DND-192, DND-167374425
LysoSensor Green DND-153, DND-189442505
LysoSensor Yellow/Blue DND-160 (PDMPO)384 (low pH)540 (low pH)
LysoSensor Yellow/Blue DND-160 (PDMPO)329 (high pH)440 (high pH)
LysoTracker Green504511
LysoTracker Red577592
LysoTracker Yellow534551
Magdala Red524600
Magnesium Green indicator506531
Magnesium Orange550575
Marina Blue dye365460
Maxilon Brilliant Flavin 10 GFF450495
Maxilon Brilliant Flavin 8 GFF460495
Mithramycin450570
MitoTracker Green FM490516
MitoTracker Orange CMTMRos551576
MitoTracker Red CMXRos578599
Monobromobimane §394490
MPS (Methyl Green Pyronine Stilbene)364395
NBD465535
NBD amines465535
NeuroTrace 500/525 green-fluorescent Nissl stain †500525
Nile red ‡549628
Nitrobenzoxadidole460-470510-650
Noradrenaline340490-520
Nuclear Fast Red289-530580
Nuclear Yellow365495
Nylosan Brilliant Flavin E8G460510
Oregon Green 488 dye and Oregon Green 488 BAPTA indicators496524
Oregon Green 488 fluorophore496524
Oregon Green 500 fluorophore503522
Oregon Green 514 dye511530
Oregon Green 514 fluorophore511530
Pacific Blue dye410455
Pacific Orange dye400551
Pararosaniline (Feulgen)570625
Phorwite AR Solution360430
Phorwite BKL370430
Phorwite Rev380430
Phorwite RPA375430
Phosphine 3R465565
Phosphine R480-565578
Pontochrome Blue Black535-553605
POPO-1, PO-PRO-1 †434456
Primuline410550
Procion Yellow470600
Propidium iodide †536617
Pyronine410540
Pyronine B540-590560-650
Pyrozal Brilliant Flavin 7GF365495
Quinacrine Mustard423503
Resorufin571585
RH 414 ‡500635
Rhod-2 indicator552578
Rhodamine 110496520
Rhodamine 123507529
Rhodamine 5 GLD470565
Rhodamine 6G526555
Rhodamine B540625
Rhodamine B 200523-557595
Rhodamine B Extra550605
Rhodamine BB540580
Rhodamine BG540572
Rhodamine Green dye502527
Rhodamine Red dye570590
Rhodamine WT530555
Rhodol Green fluorophore499525
Rose Bengal540550-600
ROX dye580605
R-phycoerythrin565575
Serotonin365520-540
Sevron Brilliant Red 2B520595
Sevron Brilliant Red 4G500583
Sevron Brilliant Red B530590
Sevron Orange440530
Sevron Yellow L430490
SITS (Primuline)395-425450
SITS (Stilbene Isothiosulphonic Acid)365460
Snarf 1563639
Sodium Green indicator507535
Stilbene335440
Sulpho Rhodamine B Can C520595
Sulpho Rhodamine G Extra470570
SYTO 17 red fluorescent nucleic acid stain621634
SYTO blue-fluorescent nucleic acid stains 40, 41, 42, 43 †428 ± 6454 ± 13
SYTO blue-fluorescent nucleic acid stains 44, 45 †450 ± 5478 ± 7
SYTO green-fluorescent nucleic acid stains 11, 14, 15, 20, 22, 25 †515 ± 7543 ± 13
SYTO green-fluorescent nucleic acid stains 12, 13, 16, 21, 23, 24 †494 ± 6515 ± 7
SYTO orange-fluorescent nucleic acid stains 80, 81, 82, 83 †537 ± 7552 ± 8
SYTO orange-fluorescent nucleic acid stains 84, 85 †567583
SYTO red-fluorescent nucleic acid stains 17, 59, 61, 64 †615 ± 15632 ± 13
SYTO red-fluorescent nucleic acid stains 60, 62, 63 †655 ± 3675 ± 3
SYTOX Blue nucleic acid stain †445470
SYTOX Green nucleic acid stain †504523
SYTOX Orange nucleic acid stain †547570
Tetracycline390560
Tetramethylrhodamine, Rhodamine B555580
Texas Red596615
Texas Red and Texas Red-X dye595615
Thiazine Red R510580
Thioflavin 5430550
Thioflavin S430550
Thioflavin TCN350460
Thiolyte370-385477-484
Thiozol Orange453480
Tinopol CBS390430
TOTO-1, TO-PRO-1 †514533
TOTO-3, TO-PRO-3 †642660
TRITC (Tetramethyl Rhodamine Isothiocyanate)557576
True Blue365420-430
Ultralite656678
Uranine B420520
Uvitex SFC365435
X-rhod-1 indicator580602
X-Rhodamine580605
XRITC582601
Xylene Orange546580
YOYO-1, YO-PRO-1 †491509
YOYO-3, YO-PRO-3 †612631
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